The kit consists of seven different ion exchange media. The columns are delivered with a stopper at the inlet and a snapoff end at the outlet. A scale up was performed to reproduce the purification step with the fraction that demonstrated antiproliferative activity. Hitraptm iex selection kit offers a fast, simple, and convenient way to decide which ion exchanger or ion exchange ligand is best for a given application. Save time submit your details once and make multiple inquiries. With the use of the fplc, one can separate and characterize protein mixture as well as pure proteins. Hitrap capto iex selection kit is designed for screening the optimal capto ion exchange or ge multimodal chromatography resin prior to scaleup. It was shown that the investigated mab can optimally be captured by bindandelute mode cex at ph 4. Other ligands can be coupled through hydroxyl, amino or thiol groups. Documentation is easy as result files contain a complete record of a run, including method, curve data and run. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. Check there is sufficient volumes of buffers, mq and etoh 20% for the coming runs. Selection kit for iex resins in hitrap columns cytiva formerly ge.
Selection of better resolving columns improved resolution and integration of the challenging separation new system suitability test solution was incorporated into method revision product understanding allowed justification of distinct shelflife specification for pre main peak. Iex is a binding technique, independent of sample volume. Q sepharose fast flow and sp sepharose fast flow are also available. Epoxyactivated sepharose 6b epoxyactivated sepharose 6b is a preactivated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to sepharose 6b via a atom hydrophilic spacer arm order product. Hitrap capto iex selection kit ge healthcare, 28934388. Principle of ion exchange chromatography ion exchange iex chromatography can separate molecules. Protein and peptide purification hebrew university of. Hitrap iex selection kits contain sepharose fast flow and sepharose xl in combination with seven different ion exchange ligands. Select steepest gradient to give acceptable resolution at. Data file 18117288 ab ion exhange chromatography q. Ge healthcare hitrap capto iex selection kit hitrap capto iex selection kit packings for chromatography columns chromatography. Supply of iex column and matrix 1 68 19 p item iex tender.
Media selection hitraptm iex selection kit, including seven different iex media, is used for fast screening of iex ligands and for method optimization. Ion exchange chromatography iex separates proteins with differences in surface charge to give highresolution separation with high sample loading capacity. Thomas scientific is the premier online source for lab supplies, equipment, instruments and chemicals all shipped direct to you quickly and safely. Download free ge user manuals, owners manuals, instructions, warranties and installation guides, etc. Epoxyactivated sepharose 6b is a preactivated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to. Hitrap iex selection kit is used for screening of the optimal ge ion exchange resin for specific application and development work. Hitrap sp hp, 1 ml and 5 ml hitrap q hp, 1 ml and 5 ml. The media are prepacked in readytouse hitrap 1 ml columns fig 1.
Hitrap iex selection kit 17600233 hitrap q hp 17115401 hitrap butyl ff 175701 mono s 550gl 17516801. Ge hitrap q hp users manual page 18 free pdf download. Ge healthcare hitrap capto iex selection kit fisher scientific. Hitrap iex selection kit hitrap sp ff, 1 ml hitrap cm ff, 1 ml hitrap q ff, 1 ml hitrap deae ff, 1 ml hitrap anx ff high sub, 1 ml hitrap q xl, 1 ml hitrap sp xl, 1 ml hitrap iex selection kit consists of seven hitrap 1 ml columns, prepacked with different ion exchangers. Hitrap protein ag, histrap ff crude, hitrap talon superdex, mono s, mono q, resource, capto qs, iex hic selection kits prepacked columns hitrap can use without system, hiscreen, hiprep. Using technology similar to a water softenertrace amounts of minerals or other contamination are condensate polishing unit from the system before such contamination becomes concentrated enough to cause condensate polishing unit by depositing minerals inside pipes, or within precisionengineered devices condensate polishing unit as boilerssteam generatorsheat. Hitrap capto ion exchange selection kit cytiva formerly ge. Ion exchange columns and media selection guide imagination at work. See also ion exchange columns and media guide, 18112731. Hitrap iex selection kit 17600233 hitrap capto iex selection kit 28934388 more on page 10. Sec size exclusion chromatography iex ion exchange chromatography ac affinity chromatography ge format guide type of purification. Data file 18114048 ae ion exchange chromatography hitrap.
Hitrap capto iex selection kit offers a fast, simple. The hitrap hic selection kit consists of seven hydrophobic interaction chromatography hic media with different hydrophobic characteristics. Table 1 lists the characteristics of hitrap columns. Media and column selection refer to ion exchange selection guide code no. Federal government and provides supplies and equipment to over 25 agencies including the center for disease control, the food and drug administration, health and. A chromatographic matrix containing hydrophobic groups, binds proteins from aqueous solutions to different extents depending on the protein structures and a range of controllable factors including concentrations of salts, ph, temperature and organic solvents fig 2. Click on the chromatography method you are interested in to jump to the corresponding selection guide. The developed phgradient iex was applied as an analytical tool for the selection of phrelated conditions in an ionexchange chromatographic capture of a mab from hybridoma ccs.
Epoxyactivated sepharose 6b ge healthcare life sciences. The kit consists of seven different ion exchange media prepacked in hitrap 1 ml columns. Ge healthcare hitrap capto iex selection kit hitrap capto. Related resins available in hitrap iex selection kit although it does not include q or sp sepharose high performance resins, the hitrap iex selection kit will be of interest to many potential users of these two ion exchangers. The separation is based on the reversible interaction between a charged protein and an oppositely charged chromatography resin. Hitrapconvenient protein purification fisher scientific. Hitrap columns, capto iex selection kit, ge healthcare vwr.
The five hitrap 1 ml columns are also included in hitrap iex selection kit together with sp sepharose xl and q sepharose xl. Use small prepacked columns for media scouting and method optimization, to increase efficiency in method development e. Related media available in hitrap iex selection kit although it does not include q or sp sepharose high performance media, the hitrap iex selection kit will be of interest to many potential users of these two ion exchangers. The kit provides you with the possibility to screen for the most appropriate hic media to use for specific application and development work. Read and download ge dishwasher hitrap q hp users manual page 18 online.
Property technique size gel filtration gf, also called size exclusion charge ion exchange chromatography iex hydrophobicity hydrophobic i nteraction chromatography hic. Sample preparation samples should be at the same ph and ionic strength as the starting buffer, and free from. Ge healthcare hitrap iex selection kit includes seven 1ml iex. Purification antibody purification handbook 18103746 gel. Combine chromatography techniques to optimize your protein. Designed for use with syringe, peristalic pump, or chromatography systems such as akta design for convenient media screening and optimization studies. Biomolecules are purified using chromatography techniques that separate them according to differences in their specific properties, as shown in figure 1. Ion exchange columns and media hebrew university of. The kit consists of seven different ion exchange resins prepacked in hitrap 1 ml.
Ge healthcare your guide to chromatography media pure proteins today. Prepacked columns from ge healthcare will ensure reproducible results and the highest performance. Ge healthcare hitrap iex selection kit includes seven 1ml. Microsoft powerpoint purifier instructional material. The seven different hic media are prepacked in ready to use 1 ml hitrap columns. Use hitrap iex selection kit for media scouting and method optimization. Hitrap iex selection kit ge healthcare, 17600233 sigmaaldrich. Hitrap column characteristics the columns are made of biocompatible polypropylene that does not interact with biomolecules. The kit consists of seven different ion exchange media based on sepharose fast flow and sepharose xl. Hitrap iex selection kit offers a fast, simple, and convenient way to decide which ion exchanger or ion exchange ligand is best for a given application. Thomas scientific is a registered contractor for the u.
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